Fluorescent Probes in DNA and RNA

A precise understanding of gene expression in living organisms is the goal of various research disciplines. Fluorescence microscopy is an essential imaging tool for observing these processes. Hybridisation-sensitive probes that selectively bind to target sequences are used for this purpose. The change in their fluorescence properties enables dynamic, non-invasive observation of cellular processes.

Our working group specialises in the development of suitable fluorophores and their covalent incorporation into oligonucleotides. The fluorophores are either incorporated directly as phosphoramidites during DNA/RNA solid-phase synthesis, or the DNA/RNA is modified post-synthetically, for example using bioorthogonal labelling methods such as click chemistry.

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