# 1 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
;CLAP_hsqcetgp
;
; Pulse Sequence downloaded from http://www.ioc.kit.edu/luy/
;
; This pulse sequence may contain errors and is intended for information only.
; Use on a spectrometer is at your own risk.
; Additions to the Bruker hsqcetgp sequence are copyright Karlsruhe Institute of Technology (KIT) 2013.
;
; By using this sequence, or any modification of it, you agree to acknowledge the website 
; "http://www.ioc.kit.edu/luy/" in any resulting publication, and cite the paper
; A. Enthart, J.C. Freudenberger, J. Furrer, H. Kessler, B. Luy, 
; The CLIP/CLAP-HSQC: Pure absorptive spectra for the measurement of one-bond couplings,
; J. Magn. Reson. 192, 314-322 (2008).
;
;based on hsqcetgp
;avance-version (09/04/17)
;HSQC
;2D H-1/X correlation via double inept transfer
;phase sensitive using Echo/Antiecho-TPPI gradient selection
;
;$CLASS=HighRes
;$DIM=2D
;$TYPE=
;$SUBTYPE=
;$COMMENT=


# 1 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/Avance.incl" 1
;Avance2.incl
;   for TCU3
;
;avance-version (07/08/21)
;
;$CLASS=HighRes Incl
;$COMMENT=
# 141 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/Avance.incl"
;$Id: Avance2.incl,v 1.23 2009/07/23 15:57:04 ber Exp $
# 28 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp" 2

# 1 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/Grad.incl" 1
;Grad2.incl  -  include file for Gradient Spectroscopy
;   for TCU3
;
;avance-version (07/01/17)
;
;$CLASS=HighRes Incl
;$COMMENT=

# 27 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/Grad.incl"
define list<gradient> EA=<EA>


# 31 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/Grad.incl"
;$Id: Grad2.incl,v 1.13 2009/07/02 16:40:43 ber Exp $
# 29 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp" 2

# 1 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/Delay.incl" 1
;Delay.incl  -  include file for commonly used delays
;
;version 00/02/07
;
;$CLASS=HighRes Incl
;$COMMENT=

# 9 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/Delay.incl"
;general delays

define delay DELTA
define delay DELTA1
define delay DELTA2
define delay DELTA3
define delay DELTA4
define delay DELTA5
define delay DELTA6
define delay DELTA7
define delay DELTA8

define delay TAU
define delay TAU1
define delay TAU2
define delay TAU3
define delay TAU4
define delay TAU5
# 29 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/Delay.incl"
;delays for centering pulses

define delay CEN_HN1
define delay CEN_HN2
define delay CEN_HN3
define delay CEN_HC1
define delay CEN_HC2
define delay CEN_HC3
define delay CEN_HC4
define delay CEN_HP1
define delay CEN_HP2
define delay CEN_CN1
define delay CEN_CN2
define delay CEN_CN3
define delay CEN_CN4
define delay CEN_CP1
define delay CEN_CP2

# 48 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/Delay.incl"
;loop counters

define loopcounter COUNTER
define loopcounter SCALEF
define loopcounter FACTOR1
define loopcounter FACTOR2
define loopcounter FACTOR3

# 58 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/Delay.incl"
;$Id: Delay.incl,v 1.13 2009/07/02 16:40:43 ber Exp $
# 30 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp" 2


# 33 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
"p2=p1*2"
"p4=p3*2"
"d4=1s/(cnst2*4)"
"d11=30m"


# 44 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
"acqt0=0"

"d0=3u"

"in0=inf1/2"
# 51 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
"DELTA1=d4-p16-de+p1*2/PI-8u"


# 56 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
"DELTA=p16+d16+p2+d0*2"


# 1 "mc_line 60 file C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp exp. def. part of mc cmd. before ze"
define delay MCWRK
define delay MCREST
define delay d0orig
"d0orig=d0"
define loopcounter t1loop
"t1loop=0"
define loopcounter ph1loop
"ph1loop=0"
define loopcounter ST1CNT
"ST1CNT = td1/(2)"
"MCWRK = 0.090909*d1"
"MCREST = d1 - d1"

    dccorr
# 60 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
# 60 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
1 ze
# 1 "mc_line 60 file C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp exp. def. of mc cmd. after ze"
# 61 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
  d11
# 1 "mc_line 62 file C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp exp. start label for mc cmd."
2 MCWRK  * 5
LBLF1, MCWRK  * 5
LBLST1, MCWRK
  MCREST
# 63 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
3 (p1 ph1)
  d4 pl2:f2
  (center (p2 ph1) (p4 ph6):f2 )
  d4 setnmr3|0 setnmr0|34|32|33 ctrlgrad 0
  (p1 ph2) (p3 ph3):f2
  d0 


# 73 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
  (p2 ph5)


  d0
  p16:gp1*EA
  d16
  (p4 ph4):f2
  DELTA
  (ralign (p1 ph1) (p3 ph4):f2 )
  d4
  (p2 ph1)
  4u
  p16:gp2
  DELTA1
  4u setnmr3^0 setnmr0^34^32^33 ctrlgrad 7
  go=2 ph31
# 1 "mc_line 89 file C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp exp. mc cmd. in line"
  MCWRK  wr #0 if #0 zd 

  "ph1loop+=1"
      "d0=d0orig + t1loop * in0 "
      "phval3 = (t1loop * 180)%360"
      MCWRK ip3 + phval3
      "phval6 = (t1loop * 180)%360"
      MCWRK ip6 + phval6
      "phval31 = (t1loop * 180)%360"
      MCWRK ip31 + phval31
      "phval0 = ph1loop * 1"
      MCWRK setgrad EA
  lo to LBLF1 times 2
  MCWRK 

  "t1loop+=1"
      "d0=d0orig + t1loop * in0 "
      "phval3 = (t1loop * 180)%360"
      MCWRK ip3 + phval3
      "phval6 = (t1loop * 180)%360"
      MCWRK ip6 + phval6
      "phval31 = (t1loop * 180)%360"
      MCWRK ip31 + phval31
      "phval0 = ph1loop * 1"
      MCWRK setgrad EA
  lo to LBLST1 times ST1CNT
  MCWRK 
  "t1loop=0"
  "ph1loop=0"
  MCWRK
# 91 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
exit
   
# 94 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
ph1=0 
ph2=1
ph3=0 2
ph4=0 0 0 0 2 2 2 2
ph5=0 0 2 2
ph6=0
ph31=1 3 1 3 3 1 3 1
  

;pl1 : f1 channel - power level for pulse (default)
;pl2 : f2 channel - power level for pulse (default)
;pl3 : f3 channel - power level for pulse (default)
;pl12: f2 channel - power level for CPD/BB decoupling
;p1 : f1 channel -  90 degree high power pulse
;p2 : f1 channel - 180 degree high power pulse
;p3 : f2 channel -  90 degree high power pulse
;p4 : f2 channel - 180 degree high power pulse
;p16: homospoil/gradient pulse
;p22: f3 channel - 180 degree high power pulse
;p28: f1 channel - trim pulse
;d0 : incremented delay (2D)                  [3 usec]
;d1 : relaxation delay; 1-5 * T1
;d4 : 1/(4J)XH
;d11: delay for disk I/O                             [30 msec]
;d16: delay for homospoil/gradient recovery
;cnst2: = J(XH)
;inf1: 1/SW(X) = 2 * DW(X)
;in0: 1/(2 * SW(X)) = DW(X)
;nd0: 2
;NS: 1 * n
;DS: >= 16
;td1: number of experiments
;FnMODE: echo-antiecho
;cpd2: decoupling according to sequence defined by cpdprg2
;pcpd2: f2 channel - 90 degree pulse for decoupling sequence

# 131 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
;use gradient ratio:	gp 1 : gp 2
;			  80 : 20.1    for C-13
;			  80 :  8.1    for N-15

;for z-only gradients:
;gpz1: 80%
;gpz2: 20.1% for C-13, 8.1% for N-15

;use gradient files:   
;gpnam1: SMSQ10.100
;gpnam2: SMSQ10.100
# 144 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
                                          ;preprocessor-flags-start
;LABEL_CN: for C-13 and N-15 labeled samples start experiment with 
;             option -DLABEL_CN (eda: ZGOPTNS)
                                          ;preprocessor-flags-end


# 151 "C:/Bruker/TopSpin3.1/exp/stan/nmr/lists/pp/user/CLAP_hsqcetgp"
;$Id: hsqcetgp,v 1.5.4.1 2011/02/24 17:26:40 ber Exp $
