README File for the CLIP-COSY experiment

The archive contains two experiment folders based on experiments on Glucose in D2O that were run using Bruker TopSpin 3.2: 
a CLIP-COSY with hard (rectangular) pulses in the folder 10 and 
a CLIP-COSY with shaped pulses in the folder 20. 

The pulse programs for the two experiments and the shapes required for normal proton experiments are included alongside. The pulse programs and shaped files have to be copied in the appropriate directories (.../exp/stan/nmr/pp/user/ and .../exp/stan/nmr/wave/user/, respectively).
The experiments were acquired on a 600 MHz spectrometer with a TCI probehead with 50 Gs/cm z-gradients. You may have to change the gradient strength GPZ1 and 2 if Your gradient strength differs. To be absolutely sure, You can go through the instructions from the Keeler group for setting up proper values for the z-filters (http://www-keeler.ch.cam.ac.uk/publications/supporting%20info.pdf). The SmChirp-shapes for the z-filter were created for a 40 kHz band width just covering the typical proton spectrum in addition to the spectral broadening due to the z-gradient. The pulse length of corresponding shapes is given in the file name before the last underscore (in ms).
The RF amplitude (or Rabi frequency) is specified in the file name after the last underscore (in kHz, before RF). The most simple way to set them up, is to fill the powerlevel PLW1 and the pulse length P1 with correct values for the sample and then use the au program ‘pulse’ and copy the new powerlevel to SPW23 and 24. In the data set we used ‘pulse 1.3 khz’ for SPW23 and ‘pulse 1.56 khz’  for SPW24, as the pulse length for shape 24 was reduced from 20 ms to 16.667 ms to avoid possible repetition artifacts.
In the CLIP-COSY with shaped pulses, all pulses are optimized for 20 kHz (except for the z-filter pulses). When setting up the experiment on your spectrometer, all SPW?? values need to be corrected. ‘pulse 20 khz’ will give You the right level. If the 90° hard pulse on your spectrometer/probehead combination is longer than 12.5 µs, pulse shapes have to be scaled accordingly, leading to smaller effective bandwidths. 

