An important application of high resolution NMR spectroscopy is the structure determination of organic molecules in solution, i.e. constitution, configuration and conformation. A number of sophisticated methods based on classical NMR parameters like chemical shifts, J-couplings and nuclear Overhauser enhancement (NOE) has been developed to accomplish this tasks. The approach has been successfully applied to a vast number of molecules including biomacromolecules and natural and synthetic products. A drawback of these standard NMR parameters is their short range.
The free molecular tumbling in solution effectively averages all anisotropic parameters to zero. If partial alignment is introduced full averaging cannot take place and some residual anisotropic parameters can be detected. The most widely used are the so called Residual Dipolar Couplings (RDCs). They depend on the distance between interacting nuclei and on the angle between internuclear vector and external magnetic field. Thus, RDCs contain structural information about the molecule in a wider range. Our work is focused on development of gels suitable for alignment media and the structure evaluation with the measured RDCs. ... See more.