;bulusgtrosy ;avance-version ;Single scan Gradient enhanced TROSY ;J. Weigelt, JACS 120, 10778 (1998) ;with non-selective JE-TROSY approach incorporated ;B. Luy, J. P. Marino, JMR 163, 92-98 (2003). ;selective option is incorporated as comment. #include #include "p2=p1*2" "p4=p3*2" "p6=p5*2" "p16=1m" "d0=6u" "d10=6u" "d16=200u" "d4=2.7m-8u" "d26=(p6-p2)/2" "d25=(p5-p1)/2" ;"d27=(p10-p6)/2" ;selective version 1 ze 2 10m 3 10m 4 10m 5 10m 6 d1 do:f2 8u UNBLKGRAD 4u pl1:f1 pl3:f3 (p1 ph10):f1 d4 gron0 d0 2u groff (d26 p2 ph0):f1 (p6 ph9):f3 ;non-selective version ; 4u ;selective version ; (d27 p6 ph9):f3 (p10:sp1 ph0):f1 ;selective version ; 4u ;selective version d4 gron0 d0 2u groff (d25 p1 ph1):f1 (p5 ph6):f3 d10 gron0 2u groff 18u d10 gron0*-1 2u groff 20u p16:gp1*EA d16 (p6 ph0):f3 20u p16:gp10*EA d16 (p1 ph4):f1 d4 p16:gp2 d16 (d26 p2 ph1):f1 (p6 ph0):f3 d4 p16:gp2 d16 (d25 p1 ph2):f1 (p5 ph1):f3 d4 p16:gp3 d16 (d26 p2 ph0):f1 (p6 ph0):f3 d4 p16:gp3 d16 (p5 ph5):f3 4u p16:gp11 d16 p27*0.231 ph7 d19*2 p27*0.692 ph7 d19*2 p27*1.462 ph7 d19*2 p27*1.462 ph8 d19*2 p27*0.692 ph8 d19*2 p0*0.231 ph8 4u p16:gp12 d16 BLKGRAD go=2 ph31 30m do:f2 wr #0 if #0 zd 3m id0 lo to 3 times l4 3m rd0 3m ip4 3m ip4 3m ip5 3m ip5 3m igrad EA lo to 5 times 2 3m ip6 3m ip6 3m ip9 3m ip9 3m ip31 3m ip31 3m id10 lo to 6 times l3 exit ph0=0 ph1=1 ph2=2 ph3=3 ph4=1 ph5=0 ph6=1 3 ph7=0 0 2 2 ph8=2 2 0 0 ph9=0 ph10=0 ph31=0 2 ;pl1 : f1 channel - power level for pulse (default) ;pl2 : f2 channel - power level for pulse (default) ;pl3 : f3 channel - power level for pulse (default) ;p1 : f1 channel - 90 degree high power pulse ;p3 : f2 channel - 90 degree high power pulse ;p5 : f3 channel - 90 degree high power pulse ;d1 : relaxation delay; 1-5 * T1 ;Gradients as published in J. Weigelt, JACS. ;We used: ; gp1 : gp2 : gp3 : gp10 : gp11 : gp12 ; 75 : 7.5 : 7.5 : -75 : 15 : 30.25 ; ;Different gradient strengths might yield better results depending on the ;spectrometer and probehead.