;HMBC ;avance-version ;2D H-1/X correlation via heteronuclear zero and double quantum ; coherence ;optimized on long range couplings ;no decoupling during acquisition ;with optimized pulses on 13C-channel #include #include define list EA1 = { cnst22 cnst23 } define list EA2 = { -cnst23 -cnst22 } "d4=1/4*cnst4" "d5=1/2*cnst5" 1 ze 2 d1 3 d11 pl2:f2 d11 pl1:f1 4 p1 ph0 d4 UNBLKGRAD (center (p2 ph0):f1 (p14:sp14 ph5):f2) d4 (lalign (p1 ph0):f1 (p13:sp13 ph5):f2) 5u p16:gp1*0.3333 d16 p1 ph1 d5 (p13:sp13 ph3):f2 d0 p2 ph2 d0 3u p16:gp1*EA1 d16 (p14:sp14 ph5):f2 3u p16:gp1*EA2 d16 (p15:sp15 ph4):f2 4u BLKGRAD go=2 ph31 d1 mc #0 to 2 F1EA(igrad EA1 & igrad EA2, id0 & ip3*2 & ip31*2) exit ph0=0 ph1=0 0 0 0 2 2 2 2 ph2=0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 ph3=1 3 ph4=3 3 1 1 ph5=3 ph31=0 2 2 0 2 0 0 2 2 0 0 2 0 2 2 0 ;pl1 : f1 channel - power level for pulse (default) ;pl2 : f2 channel - power level for pulse (default) ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p3 : f2 channel - 90 degree high power pulse ;p13 : f2 channel - 90 degree optimized Iz->Ix pulse ;p15 : f2 channel - 90 degree optimized Ix->Iz pulse ;p14 : f2 channel - 180 degree optimized pulse ;d0 : incremented delay (2D) ;d1 : relaxation delay; 1-5 * T1 ;d11 : power switching delay; ;d5 : HMBC delay (normally 1/(2J)XH long range) ;cnst4 : 1J CH ;cnst5 : long-range CH coupling ;in0: 1/(2 * SW(X)) = DW(X) ;nd0: 2 ;NS: 2 * n ;DS: 16 ;td1: number of experiments ; cnst22 cnst23 ; +0.5 -0.3 ; +0.3 -0.5